Difference between revisions of "Part:BBa K2271116"

 
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<partinfo>BBa_K2271116 short</partinfo>
 
<partinfo>BBa_K2271116 short</partinfo>
  
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The ADH we used for our project is from Pichia pastoris. It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Usually an additionally cofactor regeneration is not necessary, as BM3 degrades NADH+H+ into NAD+ and ADH regenerates it. But because of the improved (+)-nootkatone producing function of BM3, another cofactor regenerating enzyme is necessary. In recent studies it was shown that Glucose Dehydrogenases (GDH) are appropriate enzymes for this attempt.
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This part additionally holds a PTS1 sequence for peroxisomal PEX5 import. For cytosolic expression please choose part: BBa_K2271115
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:04, 30 October 2017


ADH PTS1

The ADH we used for our project is from Pichia pastoris. It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Usually an additionally cofactor regeneration is not necessary, as BM3 degrades NADH+H+ into NAD+ and ADH regenerates it. But because of the improved (+)-nootkatone producing function of BM3, another cofactor regenerating enzyme is necessary. In recent studies it was shown that Glucose Dehydrogenases (GDH) are appropriate enzymes for this attempt.

This part additionally holds a PTS1 sequence for peroxisomal PEX5 import. For cytosolic expression please choose part: BBa_K2271115

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 2047
    Illegal XhoI site found at 2083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2433
  • 1000
    COMPATIBLE WITH RFC[1000]