Difference between revisions of "Part:BBa K2207025"
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Modified Promoter T7 | Modified Promoter T7 | ||
| − | This part | + | This part contains a T7 promoter derived from <partinfo>K525998</partinfo> with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene. |
| − | Usage and Biology | + | <b>Usage and Biology</b> |
We put the Biobrick E1010(mRFP) behind the K2207025 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207025 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below. | We put the Biobrick E1010(mRFP) behind the K2207025 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207025 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below. | ||
| + | |||
| + | https://static.igem.org/mediawiki/2017/2/2a/ZJU_China_R_25.png | ||
According to the data, it is easy to find that the ratio of relative strength of K2207025 and K525998 is 11.44, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter. | According to the data, it is easy to find that the ratio of relative strength of K2207025 and K525998 is 11.44, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter. | ||
Latest revision as of 15:01, 30 October 2017
Modified T7 promoter 2
BBa_K2207025
Modified Promoter T7
This part contains a T7 promoter derived from BBa_K525998 with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
Usage and Biology
We put the Biobrick E1010(mRFP) behind the K2207025 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207025 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.
According to the data, it is easy to find that the ratio of relative strength of K2207025 and K525998 is 11.44, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]