Difference between revisions of "Part:BBa K2207025"

 
 
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<partinfo>BBa_K2207025 short</partinfo>
 
<partinfo>BBa_K2207025 short</partinfo>
  
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We induced site mutation into T7 promoter to build up a series of promoters of different strengths.
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BBa_K2207025
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Modified Promoter T7
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This part contains a T7 promoter derived from <partinfo>K525998</partinfo> with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
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<b>Usage and Biology</b>
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We put the Biobrick E1010(mRFP) behind the K2207025 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207025 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.
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https://static.igem.org/mediawiki/2017/2/2a/ZJU_China_R_25.png
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According to the data, it is easy to find that the ratio of relative strength of K2207025 and K525998 is 11.44, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:01, 30 October 2017


Modified T7 promoter 2

BBa_K2207025

Modified Promoter T7

This part contains a T7 promoter derived from BBa_K525998 with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.

Usage and Biology

We put the Biobrick E1010(mRFP) behind the K2207025 and K525998 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2207025 and wild-type T7 promoter(K525998) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.

ZJU_China_R_25.png

According to the data, it is easy to find that the ratio of relative strength of K2207025 and K525998 is 11.44, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]