Difference between revisions of "Part:BBa K2257007"

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FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.<br/>
 
FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.<br/>
 
Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.</p>
 
Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.</p>
 +
[[File:T-Nanjing-China-ch2o-l2.png|600px|thumb|center]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:20, 30 October 2017


This part is used for the detection of formaldehyde.

Formaldehyde sensor sequence


Usage and Biology

This protein FrmR acts as regulator of protein FrmAB. FrmA and FrmB are two proteins that are participating in the detoxication of formaldehyde in E.coli.
FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.
Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.

T-Nanjing-China-ch2o-l2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 226
    Illegal XhoI site found at 462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 520

The sequence is composed of PfrmR, gene frmR, flag tag, PfrmAB, gene RFP.
When the pathway works, we can see that the E.coli turns to red with naked eyes at the presence of formaldehyde.
The fluorescence intensity of protein RFP and the depth of the color in naked eyes are both in positive correlation with the concentration of formaldehyde within the tolerable range.