Difference between revisions of "Part:BBa K2323008"

 
 
(One intermediate revision by the same user not shown)
Line 4: Line 4:
  
  
−
pTet promoter, RBS B0034, GFP E0040, pdt2B degradation tag, Terminator B0015 This GFP is tagged by an pdt2B degradation tag to be recognized by ClpX protease from E.coli  
+
This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2B. This causes the protein to degrade in E.coli with a first-order rate of 0.0069 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.
 +
 
 +
<!-- -->
 +
This biobrick is part of a collection of degradation tags, characterized on the page [https://parts.igem.org/Part:BBa_K2323003 BBa_K2323003]. See also [https://parts.igem.org/Part:BBa_K2323005 BBa_K2323005], [https://parts.igem.org/Part:BBa_K2323006 BBa_K2323006], [https://parts.igem.org/Part:BBa_K2323007 BBa_K2323007].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 13: Line 16:
 
<partinfo>BBa_K2323008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2323008 SequenceAndFeatures</partinfo>
  
−
 
+
===Plasmid composition===
 +
[[File:BBa_K2323008.png| frame | 400px | center | The collection is cloned from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. ([https://benchling.com/s/RjXDZtjN read-only benchling map]) Cloning was confirmed with sequencing. ]]
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2323008 parameters</partinfo>
 
<partinfo>BBa_K2323008 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 13:49, 30 October 2017


GFP-pdt2B


This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2B. This causes the protein to degrade in E.coli with a first-order rate of 0.0069 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

This biobrick is part of a collection of degradation tags, characterized on the page BBa_K2323003. See also BBa_K2323005, BBa_K2323006, BBa_K2323007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724

Plasmid composition

The collection is cloned from BBa_I13522 with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. (read-only benchling map) Cloning was confirmed with sequencing.