Difference between revisions of "Part:BBa K2323006"

 
 
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<partinfo>BBa_K2323006 short</partinfo>
 
<partinfo>BBa_K2323006 short</partinfo>
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This GFP (E0040) is tagged with a protein degradation tag (pdt) called LAA. This causes the protein to degrade in E.coli with a first-order rate of 0.0327 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.
  
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pTet promoter, RBS B0034, GFP E0040, ASV degradation tag, Terminator B0015
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This biobrick is part of a collection of degradation tags, characterized on the page [https://parts.igem.org/Part:BBa_K2323003 BBa_K2323003]. See also [https://parts.igem.org/Part:BBa_K2323005 BBa_K2323005], [https://parts.igem.org/Part:BBa_K2323007 BBa_K2323007], [https://parts.igem.org/Part:BBa_K2323008 BBa_K2323008].
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This GFP is tagged by an LAA degradation tag to be recognized by ClpX protease from E.coli
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2323006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2323006 SequenceAndFeatures</partinfo>
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===Plasmid composition===
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[[File:BBa_K2323006.png| frame | 400px | center | The collection is cloned from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. ([https://benchling.com/s/9bY7WB1X read-only benchling map]) Cloning was confirmed with sequencing. ]]
  
  

Latest revision as of 13:42, 30 October 2017


GFP-LAA This GFP (E0040) is tagged with a protein degradation tag (pdt) called LAA. This causes the protein to degrade in E.coli with a first-order rate of 0.0327 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

This biobrick is part of a collection of degradation tags, characterized on the page BBa_K2323003. See also BBa_K2323005, BBa_K2323007, BBa_K2323008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724

Plasmid composition

The collection is cloned from BBa_I13522 with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. (read-only benchling map) Cloning was confirmed with sequencing.