Difference between revisions of "Part:BBa K2333011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | codon | + | This specific sequence was codon-optimized for E. coli using the website www.jcat.de |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | This sequence was adapted from Gur, Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". Synthesized by IDT. | |
===References=== | ===References=== | ||
| + | Cameron DE, Collins JJ. (2014) Tunable protein degradation in bacteria. Nature Biotechnology, 32(12):1276–1281. | ||
| + | |||
| + | Gur E, Sauer RT. (2008) Evolution of the ssra degradation tag in mycoplasma: specificity switch to a different protease. Proceedings of the National Academy of Sciences,105(42):16113– 16118. | ||
Latest revision as of 13:35, 30 October 2017
mf-Lon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1834
Illegal AgeI site found at 1918
Illegal AgeI site found at 2124
Illegal AgeI site found at 2149 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This specific sequence was codon-optimized for E. coli using the website www.jcat.de
Source
This sequence was adapted from Gur, Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". Synthesized by IDT.
References
Cameron DE, Collins JJ. (2014) Tunable protein degradation in bacteria. Nature Biotechnology, 32(12):1276–1281.
Gur E, Sauer RT. (2008) Evolution of the ssra degradation tag in mycoplasma: specificity switch to a different protease. Proceedings of the National Academy of Sciences,105(42):16113– 16118.