Difference between revisions of "Part:BBa K2323003:Design"
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| − | + | This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0046/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon). | |
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===References=== | ===References=== | ||
| + | Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014 | ||
Latest revision as of 13:34, 30 October 2017
GFP-pdt2E
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724
Design Notes
This protein degradation tag is part of a collection, which is easy to clone to tag any protein using overhang PCR.
Source
This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0046/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon).
References
Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014