Difference between revisions of "Part:BBa K2201273"
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| − | Reversed mRFP under T3- | + | Reversed mRFP under T3-promoter control. This part could be used as a highly specific reporter in complex genetic cirsuits. The opposite direction of the CDS prevents basal transcription. Furthermore the T3 and T7 polymerase are orthognal, thus this reporter could be used orthgonal to the expression with the T7-polyerase in <i>E. coli</i> strains like BL21. The characterisation of this part could be found on the page of the composite part BBa_K2201373. |
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Latest revision as of 13:28, 30 October 2017
Reversed mRFP under T3-polymerase control for signal strengthening
Reversed mRFP under T3-promoter control. This part could be used as a highly specific reporter in complex genetic cirsuits. The opposite direction of the CDS prevents basal transcription. Furthermore the T3 and T7 polymerase are orthognal, thus this reporter could be used orthgonal to the expression with the T7-polyerase in E. coli strains like BL21. The characterisation of this part could be found on the page of the composite part BBa_K2201373.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 147 - 1000COMPATIBLE WITH RFC[1000]