Difference between revisions of "Part:BBa K2336036"
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
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Revision as of 13:21, 30 October 2017


PmrC-GFP

This part is used to detect whether the sequence is expressed successfully.


Usage and Biology

PmrC is a promoter used to start the composite. GFP is green fluoresent protein gene.

The aim that we design this part is to get prepared for the material, then PmrA-PmrB(LBT) can be linked to it. Because we need a large amount of this part, we standardize this part independently. Actually, this part is not a functional part. It a basic to get more parts and construct the whole circuit. Figure 1-1 shows the circuit of PmrC-GFP.

Figure 1-1: The circuit of PmrC-GFP.

Characterization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 397
    Illegal XhoI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1085