Difference between revisions of "Part:BBa K2333011"

Revision as of 13:18, 30 October 2017

mf-Lon

This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1834
Illegal AgeI site found at 1918
Illegal AgeI site found at 2124
Illegal AgeI site found at 2149
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2333011 short</partinfo>
-mflon
+This part codes for the AAA+ Lon Protease from <i>Mesoplasma florum</i> bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006.
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