Difference between revisions of "Part:BBa K2323000"

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<partinfo>BBa_K2323000 short</partinfo>
 
<partinfo>BBa_K2323000 short</partinfo>
  
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Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.
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Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.  
  
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The coding sequence is followed by Tphi terminator.
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For characterization, see the full construct: [https://parts.igem.org/Part:BBa_K2323004 BBa_K2323004].
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2323000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2323000 SequenceAndFeatures</partinfo>
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<partinfo>Benchling plasmid map and sequence: https://benchling.com/s/seq-IA6dwUFOSs7Tu0prM4VE</partinfo>
 
  
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===Plasmid composition===
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[[File:BBa_K2323000.png| frame | 400px | center | The Biobrick contains the Lwa coding sequence and the Tphi terminator.([https://benchling.com/s/seq-IA6dwUFOSs7Tu0prM4VE read-only benchling map]) ]]
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The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)
  
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2323000 parameters</partinfo>
 
<partinfo>BBa_K2323000 parameters</partinfo>
 
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Latest revision as of 13:12, 30 October 2017


Lwa Cas13a + Tphi terminator

Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.

The coding sequence is followed by Tphi terminator.

For characterization, see the full construct: BBa_K2323004. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365

Plasmid composition

The Biobrick contains the Lwa coding sequence and the Tphi terminator.(read-only benchling map)

The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)