Difference between revisions of "Part:BBa K2315011"
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− | ==Fluorescent Response cognate | + | ==<b>Fluorescent Response to cognate 3OC12-HSL</b>== |
To validate the part's design, we've used standard 3OC12HSL (HSL produced by LasI in P.aeruginosa) sample to determine the response curve of this part. | To validate the part's design, we've used standard 3OC12HSL (HSL produced by LasI in P.aeruginosa) sample to determine the response curve of this part. | ||
Revision as of 13:10, 30 October 2017
Contents
LasR-GFP
Group: Shanghaitech iGEM 2017
HSL receiver from P.aeruginosa, actives expression of GFP protein in response to 3OC12HSL.
This part is one of the interchangeable AHL receivers. Full collection could be viewed at:
Introduction
(Figure1-mechanism to be added)
When proper AHL added with concentration higher than a critical value, The constitutively expressed LasR will bind the signaling AHL molecule, dimerize and bind to pLas regulatory sequence before GFP gene and actives its expression.
Fluorescent Response to cognate 3OC12-HSL
To validate the part's design, we've used standard 3OC12HSL (HSL produced by LasI in P.aeruginosa) sample to determine the response curve of this part.
(Figure2&3-response to 3OC12HSL to be added)
Orthogonality test against non-cognate inducers
(Figure4-7-response to non-cognate inducers to be added)
Usage: Measuring unknown AHL production
We developed a measurement protocol using the fluorescent protein coupled AHL receiver germs to measure the actual AHLs concentration in high precision and sensitivity in compared with LC-MS.
LasR-pLas-GFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700