Difference between revisions of "Part:BBa K2323001:Design"

 
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===References===
 
===References===
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J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)

Latest revision as of 12:55, 30 October 2017


Leptotrichia wadei Cas13a with a His-Tag and a SUMO-tag for protein purification.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1736
    Illegal BglII site found at 3416
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2815


Design Notes

This composite part includes a SUMO-Tag to improve the purification efficiency by improving solubility and allows the cleaving of the His-Tag after purification.


Source

Leptotrichia wadei

References

J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)