Difference between revisions of "Part:BBa K2315011"
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=== Rpa-Las signal converter ===
  
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__NOTOC__
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Group: <b>Shanghaitech iGEM 2017</b>
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<partinfo>BBa_K2315011 short</partinfo>
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HSL receiver from P.aeruginosa, actives expression of GFP protein in presence of 3OC12HSL.  
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==<b>Introduction</b>==
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HSL receiver from P.aeruginosa, actives expression of GFP protein in response to 3OC12HSL.  
  
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(Figure1-mechanism to be added)
  
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When proper AHL added with concentration higher than a critical value, The constitutively expressed LasR will bind the signaling AHL molecule, dimerize and bind to pLas regulatory sequence before GFP gene and actives it's expression.
  
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<partinfo>BBa_K2315011 short</partinfo>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:52, 30 October 2017

Rpa-Las signal converter

Group: Shanghaitech iGEM 2017

Introduction

HSL receiver from P.aeruginosa, actives expression of GFP protein in response to 3OC12HSL.

(Figure1-mechanism to be added)

When proper AHL added with concentration higher than a critical value, The constitutively expressed LasR will bind the signaling AHL molecule, dimerize and bind to pLas regulatory sequence before GFP gene and actives it's expression.





LasR-pLas-GFP Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1700