Difference between revisions of "Part:BBa K2440004:Design"

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===References===
 
===References===
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[1] Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T.(2004 Jul). "Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs". Mol Cell. 23;15(2):185-97. PMID: 15260970 DOI: 10.1016/j.molcel.2004.07.007
 
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[2] Hutvagner G, Simard MJ. (2008). "Argonaute proteins: key players in RNA silencing". Nature Reviews Molecular Cell Biology. 9 (1): 22–32. DOI: 10.1038/nrm2321
 
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[3] Tang G. (Feb 2005). "siRNA and miRNA: an insight into RISCs.". Trends in Biochemical Sciences. 30 (2): 106–14. PMID 15691656. DOI: 10.1016/j.tibs.2004.12.007.
 
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[4] Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T. (Jul 2004). "Human Argonaute2 Mediates RNA Cleavage Targeted by miRNAs and siRNAs". Molecular Cell. 15 (2): 185–197. PMID 15260970. DOI: 10.1016/j.molcel.2004.07.007.
 
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[5] Ronemus M, Vaughn MW, Martienssen RA. (2006 Jul). "MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis".  Plant Cell. 18(7):1559-74. Epub 2006 Jun 23. PMID: 16798886 PMCID: PMC1488920 DOI: 10.1105/tpc.106.042127
 

Latest revision as of 12:39, 30 October 2017


hAgo2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 24
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1170
    Illegal XhoI site found at 2080
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
    Illegal AgeI site found at 1868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 730


Design Notes

None.


Source

We obtain it from PCR.

References