Difference between revisions of "Part:BBa K2440008"

Revision as of 12:21, 30 October 2017

Fluc (with BsmBI restriction site)

This is the firefly luciferase reporter gene with a specific restriction site that could be recognized by BsmBI.

Usage and Biology

The firefly luciferase catalyzes a reaction that produces visible light in the 550 – 575 nm range. Numerous luc-based bioreporters have been constructed for the detection of a wide array of inorganic and organic compounds of environmental concern through the catalytic effect of the enzyme to catalysis the oxidation of firefly luciferin with the requiring of oxygen and ATP. ¹

The luminous intensity of luc is related to the substrate concentration, the experimental gene expression, so it can be used in the field of medical diagnostics.

The dual-luciferase technology means that double reporter gene is expressed simultaneously in a signal system, but independently measured by two luciferase reporter genes. One reporter gene activity is associated with specific experimental conditions for gene expression, whereas the activity of another reporter gene provides an internal control to normalize the experimental value. ²

In the pmirGLO Dual-Luciferase miRNA Target Expression Vector, the luc2 is used as the primary reporter to monitor mRNA regulation and hRluc-neo acting as a control reporter for normalization and selection.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 64
Illegal NgoMIV site found at 1408
Illegal NgoMIV site found at 1429
Illegal AgeI site found at 1132
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1314

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The firefly luciferase catalyzes a reaction that produces visible light in the 550 – 575 nm range. Numerous luc-based bioreporters have been constructed for the detection of a wide array of inorganic and organic compounds of environmental concern through the catalytic effect of the enzyme to catalysis the oxidation of firefly luciferin with the requiring of oxygen and ATP. 1.
+The firefly luciferase catalyzes a reaction that produces visible light in the 550 – 575 nm range. Numerous luc-based bioreporters have been constructed for the detection of a wide array of inorganic and organic compounds of environmental concern through the catalytic effect of the enzyme to catalysis the oxidation of firefly luciferin with the requiring of oxygen and ATP. <sup>1 </sup>
 The luminous intensity of luc is related to the substrate concentration, the experimental gene expression, so it can be used in the field of medical diagnostics.
-The dual-luciferase technology means that double reporter gene is expressed simultaneously in a signal system, but independently measured by two luciferase reporter genes. One reporter gene activity is associated with specific experimental conditions for gene expression, whereas the activity of another reporter gene provides an internal control to normalize the experimental value. 2.
+The dual-luciferase technology means that double reporter gene is expressed simultaneously in a signal system, but independently measured by two luciferase reporter genes. One reporter gene activity is associated with specific experimental conditions for gene expression, whereas the activity of another reporter gene provides an internal control to normalize the experimental value. <sup>2 </sup>
 In the pmirGLO Dual-Luciferase miRNA Target Expression Vector, the luc2 is used as the primary reporter to monitor mRNA regulation and hRluc-neo acting as a control reporter for normalization and selection.
+===Sequence and Features===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>