Difference between revisions of "Part:BBa K2323008"

 
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pTet promoter, RBS B0034, GFP E0040, pdt2B degradation tag, Terminator B0015 This GFP is tagged by an pdt2B degradation tag to be recognized by ClpX protease from E.coli  
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This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2B. This causes the protein to degrade in E.coli with a first-order rate of ... per min, from the ClpX protease. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.
  
 
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Revision as of 11:41, 30 October 2017


GFP-pdt2B


This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2B. This causes the protein to degrade in E.coli with a first-order rate of ... per min, from the ClpX protease. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724