Difference between revisions of "Part:BBa K2382003"

(→‎Usage and Biology)
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
−
This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator,
+
This part originates from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator,
 
and RBS.
 
and RBS.
  

Revision as of 10:31, 30 October 2017

T7 promoter & Lac operator and RBS from PET-29a


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

This part originates from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.

We tried to improve this promoter sequence at BBa_K525998 by adding an lac operator and making it RFC 10 compatible.

Characterization of the T7 promoter & Lac operator and RBS from PET-29a

References

pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf