Difference between revisions of "Part:BBa K2247001"

Revision as of 10:28, 30 October 2017

pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).

Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.

Characterization

Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.

This figure shows another proof of the Gal4-inducible expression from the pGalS promoter (BBa_K2247000). The above mentioned yeast strain JDYY001 was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, also validating the function of pGalS promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 453
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 54
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1130

@@ Line 14: / Line 14: @@
 ===Characterization===
+[[File:PGalS-GFP AD-BD.jpeg|left|300px]]  [[File:PGalS-GFP AD-BD-2.jpeg|center|300px]]
+=====Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.=====
+This figure shows another proof of the Gal4-inducible expression from the pGalS promoter (BBa_K2247000). The above mentioned yeast strain JDYY001 was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, also validating the function of pGalS promoter.
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