Difference between revisions of "Part:BBa K2247001"
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<partinfo>BBa_K2247001 short</partinfo>
 
<partinfo>BBa_K2247001 short</partinfo>
  
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This part encodes a GFP gene under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter
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This part encodes a GFP gene ([https://parts.igem.org/Part:BBa_K895006 BBa_K895006]) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see [https://parts.igem.org/Part:BBa_K2247000 BBa_K2247000]).
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===Usage and Biology===
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See  [https://parts.igem.org/Part:BBa_K2247000 BBa_K2247000].
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This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.
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===Characterization===
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 10:27, 30 October 2017


pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).


Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1130