Difference between revisions of "Part:BBa K2365038"
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The defensin PDC1 peptide of corn could be effectively used to restrict the Fusarium head blight mainly caused by F. graminearum. The PDC1 peptide inhibits F. graminearum growth and viability, it has potential as a control agent for the disease. | The defensin PDC1 peptide of corn could be effectively used to restrict the Fusarium head blight mainly caused by F. graminearum. The PDC1 peptide inhibits F. graminearum growth and viability, it has potential as a control agent for the disease. | ||
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<partinfo>BBa_K2365038 parameters</partinfo> | <partinfo>BBa_K2365038 parameters</partinfo> | ||
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| + | The pdc1 pcR amplification from the corn genomic DNA.Verify the target gene by Nucleic acid electrophoresis.(DL2000 DNA Marker) | ||
|[[File: Plant defensin corn 1.jpeg|300px|center]] | |[[File: Plant defensin corn 1.jpeg|300px|center]] | ||
SDS–PAGE separation of PDC1 expressed from Pichia pastoris | SDS–PAGE separation of PDC1 expressed from Pichia pastoris | ||
Revision as of 10:02, 30 October 2017
Plant defensin corn 1(pdc1)-antifungal peptide
The defensin PDC1 peptide of corn could be effectively used to restrict the Fusarium head blight mainly caused by F. graminearum. The PDC1 peptide inhibits F. graminearum growth and viability, it has potential as a control agent for the disease.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 19
Illegal NgoMIV site found at 23
Illegal NgoMIV site found at 333 - 1000COMPATIBLE WITH RFC[1000]
The pdc1 pcR amplification from the corn genomic DNA.Verify the target gene by Nucleic acid electrophoresis.(DL2000 DNA Marker)
SDS–PAGE separation of PDC1 expressed from Pichia pastoris|
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A schematic representation of our basic plasmid types, the response construct. It includes the anti-fungal peptide coding gene (anti-fungal peptide) and the inducible promoter. This construct allows genes placed downstream of the inducible promoter to be transcribed when the yeast cells are induced with the appropriate inducer—methanol.