Difference between revisions of "Part:BBa K2271066"

Revision as of 08:57, 30 October 2017

mRuby-ePTS1

Usage and Biology

This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 (Dueber et al.). The part is designed as a peroxisomal lumen marker for S. cerevisiea. Using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the optimal excitation of 559 nm and emission of 600 nm is discriped.

Experimental Design and Results

For the validation the S. cerevisiae Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1).

Figure 1 mRuby fused with PTS1 localised peroxisomal typical in the cells

Sequence and Features