Difference between revisions of "Part:BBa K876070:Experience"
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We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium. | We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium. | ||
| + | [[Image: BIT Figure OD copper 25L.png|thumb|center|500px|The OD600 values of different concentrations of part at the eighth hour.]] | ||
| + | |||
| + | [[Image: BIT-Figure-copper 25L.png|thumb|center|500px|The fluorescence values of different concentrations of part at the eighth hour.]] | ||
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 08:41, 30 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K876070
Group:iGEM_BIT2017
Author:WENJIA WANG
We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.
User Reviews
UNIQ6e678ed3416d6996-partinfo-00000000-QINU UNIQ6e678ed3416d6996-partinfo-00000001-QINU