Difference between revisions of "Part:BBa K876070:Experience"

 
 
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===Applications of BBa_K876070===
 
===Applications of BBa_K876070===
  
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'''Group:'''iGEM_BIT2017
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'''Author:'''WENJIA WANG
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We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.
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[[Image: BIT Figure OD copper 25L.png|thumb|center|500px|The OD600 values of different concentrations of part at the eighth hour.]]
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[[Image: BIT-Figure-copper 25L.png|thumb|center|500px|The fluorescence values of different concentrations of part at the eighth hour.]]
 
===User Reviews===
 
===User Reviews===
 
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Latest revision as of 08:41, 30 October 2017


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Applications of BBa_K876070

Group:iGEM_BIT2017

Author:WENJIA WANG

We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.

The OD600 values of different concentrations of part at the eighth hour.
The fluorescence values of different concentrations of part at the eighth hour.

User Reviews

UNIQ1244a03bb753acfa-partinfo-00000000-QINU UNIQ1244a03bb753acfa-partinfo-00000001-QINU