Difference between revisions of "Part:BBa K2260000:Design"
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The phaCBA operon was codon optimized for use in <i>E. coli</i>  using the <a src ="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606" codon optimization table</a>. Four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed. Furthermore, any new restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the operon.
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The phaCBA operon was codon optimized for use in <i>E. coli</i>  using the <a src ="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606"> codon optimization table</a>. Four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed. Furthermore, any new restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the operon.
 
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Revision as of 07:24, 30 October 2017


PhaCBA operon w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Four restrictions sites present in the original operon obtained from BBa_K1149051 were removed. Furthermore, any new restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the operon.

Source

The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.

References