Difference between revisions of "Part:BBa K2440006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. <sup> | + | The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. <sup>2-3 </sup> |
At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site. | At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site. | ||
Revision as of 07:08, 30 October 2017
Synthetic PA
The Poly A, also called Polyadenylation, is the addition of a poly adenine bases tail to a messenger RNA. It is important for the nuclear export, translation, and stability of mRNA. 1
Usage and Biology
The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. 2-3
At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]