Difference between revisions of "Part:BBa K2440006"

Revision as of 07:07, 30 October 2017

Synthetic PA

The Poly A, also called Polyadenylation, is the addition of a poly adenine bases tail to a messenger RNA. It is important for the nuclear export, translation, and stability of mRNA. ¹

Usage and Biology

The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. ²³

At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2440006 short</partinfo>
-Null.
+The Poly A, also called Polyadenylation, is the addition of a poly adenine bases tail to a messenger RNA. It is important for the nuclear export, translation, and stability of mRNA. <sup>1 </sup>
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The poly(A) sequence could be transcribed to be a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. It, therefore, forms part of the larger process of gene expression. <sup>23 </sup>
+At pmirGLO Vector, Synthetic polyadenylation signal is located at 2728-2776 nucleotides, with a length of 49 nucleotides. It works as the transcription stop site.
+===Sequence and Features===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>