Difference between revisions of "Part:BBa K2382004:Design"

Latest revision as of 04:41, 30 October 2017

Thioredoxin with polylinker

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 367
Illegal XhoI site found at 373
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.

And we remove the stop coden of Thioredoxin(NC_000913.3) so that it can be used in fusion protein designing.

We Insert linker between our two proteins: thioredoxine and FGD. The purpose to do so is to separate these two proteins.

The sequence of Linker contain the polylinker which has the sequence of GGTACCCGGGGATCCCTCGAGGGTGGT.

The polylinker contain four restriction sites in it:

Kpn1:GGT’ACC

Sma1: CCC’GGG

BamH1:GGA’TCC

Xho1:C’TCGAG

Two glycines are added at the end of the sequence, and it allows the protein behind linker can be reversed, reducing the probability of protein folding error.

Source

We have it synthesized by AllBio Science Incorporated.

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2382004 short</partinfo>
+===<span class='h3bb'>Sequence and Features</span>===
 <partinfo>BBa_K2382004 SequenceAndFeatures</partinfo>
 ===Design Notes===
-Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.And we remove the stop coden of Thioredoxin
+Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.
-(NC_000913.3) so that it can be used in fusion protein designing.
-We Insert linker between our two proteins: thioredoxine and MSMEG5998.
+And we remove the stop coden of Thioredoxin(NC_000913.3) so that it can be used in fusion protein designing.
-The purpose is to separate these two proteins
-The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT,
+We Insert linker between our two proteins: thioredoxine and FGD.
-There are four restriction site in it:
+The purpose to do so is to separate these two proteins.
-Kpn1:GGT’ACC
-Sma1: CCC’GGG
+The sequence of Linker contain the polylinker which has the sequence of GGTACCCGGGGATCCCTCGAGGGTGGT.
-BamH1:GGA’TCC
-Xho1:C’TCGAG
+The polylinker contain four restriction sites in it:
-The last of the sequence plus two glycine, and it allow the protein behind linker can be reversed, reducing the probability of protein folding error.
+Kpn1:'''GGT’ACC'''
+Sma1: '''CCC’GGG'''
+BamH1:'''GGA’TCC'''
+Xho1:'''C’TCGAG'''
+Two glycines are added at the end of the sequence, and it allows the protein behind linker can be reversed, reducing the probability of protein folding error.
 ===Source===
 We have it synthesized by AllBio Science Incorporated.
-Escherichia coli str. K-12 substr. MG1655
-https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3