Difference between revisions of "Part:BBa K2382007"

Revision as of 03:48, 30 October 2017

Anti-aflatoxin scFv (with start coden)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 342
Illegal SapI.rc site found at 255

Usage and Biology

And we add a start coden (ATG) This is the single chain variable fragment (scFv) of an antibody that have ability of binding aflatoxin. This part basically shares the same sequence with BBa_K2382011, except for a start codon ATG at the beginning.

Since bacteria can’t produce the whole antibody, we decide to use scFv to replace it. It contains two polypeptide chains linked by a linker.

Characterization of the Anti-aflatoxin scFv (with start coden)

References

(1) Li, X., et al., Molecular characterization of monoclonal antibodies against aflatoxins: a possible explanation for the highest sensitivity. Anal Chem, 2012. 84(12): p. 5229-35.

(2) Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357-1369. doi:10.1016/j.addr.2012.09.039

@@ Line 14: / Line 14: @@
 ===Usage and Biology===
+And we add a start coden (ATG)
+This is the single chain variable fragment (scFv) of an antibody that have ability of binding
+aflatoxin. This part basically shares the same sequence with BBa_K2382011, except for a start
+codon ATG at the beginning.
 Since bacteria can’t produce the whole antibody, we decide to use scFv to
-replace it. It contains two polypeptide chains linked by a linker. And we add a start coden (ATG)
+replace it. It contains two polypeptide chains linked by a linker.
 ===Characterization of the Anti-aflatoxin scFv (with start coden)===