Difference between revisions of "Part:BBa K2382001:Design"

 
 
(7 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2382001 short</partinfo>
 
<partinfo>BBa_K2382001 short</partinfo>
 +
===<span class='h3bb'>Sequence and Features</span>===
  
 
<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
Line 7: Line 8:
  
 
===Design Notes===
 
===Design Notes===
−
It has not been coden optimized for E. Ccoli BL21, but it seems that there has no effect on protein expression.
+
It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.  
−
 
+
Otherwise, we remove the stop coden of NC_008596.1
−
 
+
The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)
  
 
===Source===
 
===Source===
−
 
+
Mycobacterium smegmatis str. MC2 155
 
https://www.ncbi.nlm.nih.gov/nuccore/118467340/
 
https://www.ncbi.nlm.nih.gov/nuccore/118467340/
−
 
−
===References===
 

Latest revision as of 02:34, 30 October 2017


MSMEG_5998

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)

Source

Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/