Difference between revisions of "Part:BBa K2382004:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.And we remove the stop coden of Thioredoxin
+
Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.  
−
(NC_000913.3) so that it can be used in fusion protein designing.
+
 
−
We Insert linker between our two proteins: thioredoxine and MSMEG5998.
+
And we remove the stop coden of Thioredoxin(NC_000913.3) so that it can be used in fusion protein designing.
−
The purpose is to separate these two proteins
+
 
 +
 
 +
We Insert linker between our two proteins: thioredoxine and FGD.
 +
The purpose to do so is to separate these two proteins.
 +
 
 
The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT,
 
The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT,
 
There are four restriction site in it:
 
There are four restriction site in it:
 +
 
Kpn1:GGT’ACC
 
Kpn1:GGT’ACC
 +
 
Sma1: CCC’GGG
 
Sma1: CCC’GGG
 +
 
BamH1:GGA’TCC
 
BamH1:GGA’TCC
 +
 
Xho1:C’TCGAG
 
Xho1:C’TCGAG
−
The last of the sequence plus two glycine, and it allow the protein behind linker can be reversed, reducing the probability of protein folding error.
+
 
 +
Two glycine are added at the end of the sequence, and it allows the protein behind linker can be reversed, reducing the probability of protein folding error.
  
 
===Source===
 
===Source===
 
We have it synthesized by AllBio Science Incorporated.
 
We have it synthesized by AllBio Science Incorporated.

Revision as of 02:16, 30 October 2017


Thioredoxin with polylinker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.

And we remove the stop coden of Thioredoxin(NC_000913.3) so that it can be used in fusion protein designing.


We Insert linker between our two proteins: thioredoxine and FGD. The purpose to do so is to separate these two proteins.

The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT, There are four restriction site in it:

Kpn1:GGT’ACC

Sma1: CCC’GGG

BamH1:GGA’TCC

Xho1:C’TCGAG

Two glycine are added at the end of the sequence, and it allows the protein behind linker can be reversed, reducing the probability of protein folding error.

Source

We have it synthesized by AllBio Science Incorporated.