Difference between revisions of "Part:BBa K2382004:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 +
Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.And we remove the stop coden of Thioredoxin
 +
(NC_000913.3) so that it can be used in fusion protein designing.
 +
We Insert linker between our two proteins: thioredoxine and MSMEG5998.
 +
The purpose is to separate these two proteins
 +
The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT,
 +
There are four restriction site in it:
 +
Kpn1:GGT’ACC
 +
Sma1: CCC’GGG
 +
BamH1:GGA’TCC
 +
Xho1:C’TCGAG
 +
The last of the sequence plus two glycine, and it allow the protein behind linker can be reversed, reducing the probability of protein folding error.
  
 
===Source===
 
===Source===
 
We have it synthesized by AllBio Science Incorporated.
 
We have it synthesized by AllBio Science Incorporated.

Revision as of 02:10, 30 October 2017


Thioredoxin with polylinker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.And we remove the stop coden of Thioredoxin (NC_000913.3) so that it can be used in fusion protein designing. We Insert linker between our two proteins: thioredoxine and MSMEG5998. The purpose is to separate these two proteins The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT, There are four restriction site in it: Kpn1:GGT’ACC Sma1: CCC’GGG BamH1:GGA’TCC Xho1:C’TCGAG The last of the sequence plus two glycine, and it allow the protein behind linker can be reversed, reducing the probability of protein folding error.

Source

We have it synthesized by AllBio Science Incorporated.