Difference between revisions of "Part:BBa K2382004:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Thioredoxin could help the protein folding correctly, and make the fusion proteins in soluble form that are biologically active.And we remove the stop coden of Thioredoxin
 
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(NC_000913.3) so that it can be used in fusion protein designing.
 
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We Insert linker between our two proteins: thioredoxine and MSMEG5998.
 
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The purpose is to separate these two proteins
 
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The sequence of Linker contain the polylinker ,and that is: GGTACCCGGGGATCCCTCGAGGGTGGT,
 
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There are four restriction site in it:
 
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Kpn1:GGT’ACC
 
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Sma1: CCC’GGG
 
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BamH1:GGA’TCC
 
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Xho1:C’TCGAG
 
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The last of the sequence plus two glycine, and it allow the protein behind linker can be reversed, reducing the probability of protein folding error.
 
  
 
===Source===
 
===Source===
 
We have it synthesized by AllBio Science Incorporated.
 
We have it synthesized by AllBio Science Incorporated.

Revision as of 02:09, 30 October 2017


Thioredoxin with polylinker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We have it synthesized by AllBio Science Incorporated.