Difference between revisions of "Part:BBa K2271066"
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=== Experimental Design and Results=== | === Experimental Design and Results=== | ||
| − | [[File:Igemducgn2017mRuby-PTS1.png| | + | <p> For the validation the <i>S. cerevisiae</i> Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1). |
| − | + | [[File:Igemducgn2017mRuby-PTS1.png|500px|thumb|center|'''Figure 1''' mRuby fused with PTS1 localised peroxisomal typical in the cells]] </p> | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2271066 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2271066 SequenceAndFeatures</partinfo> | ||
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Revision as of 00:12, 30 October 2017
mRuby-ePTS1
Usage and Biology
The part contains a mRuby fluorezenz protein with an enhanced pts1 sequence, described by Dueber et. Al, with a TDH3 Promotor and a HHF1 Terminator. mRuby is a mutant oft he fluorezent protein gfp with excitation and emission wavelengths at 559 nm/600nm . With the C-terminal enhanced pts1 (peroxisomal targeting sequnce) the mRuby is imported into the peroxisome. The part can be used as a peroxisomal matrix marker in S. cerevisiea.
Experimental Design and Results
For the validation the S. cerevisiae Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]