Difference between revisions of "Part:BBa K2333435"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This contains mf-Lon under the control of the inducible pBad promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This arabinose-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements. | + | This part contains mf-Lon under the control of the inducible pBad promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This arabinose-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements. |
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Revision as of 20:39, 29 October 2017
pBad mf-Lon
This is an arabinose-inducible mf-Lon construct containing the pBad promoter. William and Mary 2017 has modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator.
Usage and Biology
This part contains mf-Lon under the control of the inducible pBad promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This arabinose-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1245
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1184
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1019
Illegal AgeI site found at 3102
Illegal AgeI site found at 3186
Illegal AgeI site found at 3392
Illegal AgeI site found at 3417 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1001