Difference between revisions of "Part:BBa K2333008"
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<partinfo>BBa_K2333008 short</partinfo> | <partinfo>BBa_K2333008 short</partinfo> | ||
| − | mScarlet | + | mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes. |
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| + | This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had its stop codons removed. See <partinfo>BBa_K2333009</partinfo> for this part with both stop codons. | ||
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Latest revision as of 20:26, 29 October 2017
mScarlet-I no stop codon
mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes.
This part was designed to be tagged with a protein degradation tag (see Bba_K2333001-K2333006), and as such has had its stop codons removed. See BBa_K2333009 for this part with both stop codons.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]