Difference between revisions of "Part:BBa K2271103"

Revision as of 19:36, 29 October 2017

pex15

This part contains a truncated version of the peroxisomal membrane protein pex15 (315-383). The part can be used as a peroxisomal membrane anchor on either its C- or N-terminus.

P: This part is a truncated version of the S. cerevisiae Pex15 which is a membrane peroxisomal membrane Protein. This version could be used as a membrane anchor for applications at the peroxisomal membrane. Both C- and N-Terminus could be used for a fusion. The N-Terminus is located in the cytoplasm and the C-Terminus in the peroxisomal lumen.

The functionality of this part was validated by microscopy. For the microscopy mVenus was fused to the C-Terminus of Pex15. We could observe a peroxisomal typical localisation in the cell.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
COMPATIBLE WITH RFC[1000]

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 This part contains a truncated version of the peroxisomal membrane protein pex15 (315-383). The part can be used as a peroxisomal membrane anchor on either its C- or N-terminus.
+P: This part is a truncated version of the S. cerevisiae Pex15 which is a membrane peroxisomal membrane Protein. This version could be used as a membrane anchor for applications at the peroxisomal membrane. Both C- and N-Terminus could be used for a fusion. The N-Terminus is located in the cytoplasm and the C-Terminus in the peroxisomal lumen.
+The functionality of this part was validated by microscopy. For the microscopy mVenus was fused to the C-Terminus of Pex15. We could observe a peroxisomal typical localisation in the cell.
 <!-- Add more about the biology of this part here