Difference between revisions of "Part:BBa K2382001:Design"

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Mycobacterium smegmatis str. MC2 155
 
Mycobacterium smegmatis str. MC2 155
 
https://www.ncbi.nlm.nih.gov/nuccore/118467340/
 
https://www.ncbi.nlm.nih.gov/nuccore/118467340/
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===References===
 
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(1)Lapalikar, G.V., et al., F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the Actinomycetales. PLoS One, 2012. 7(2): p. e30114.
 
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(2)Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.
 

Revision as of 16:47, 29 October 2017


MSMEG_5998


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)

Source

Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/