Difference between revisions of "Part:BBa K2446037"
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The information of other SynTF-SynPro is showed in the table below.
 
The information of other SynTF-SynPro is showed in the table below.
 +
<table border="2"  width="300px" >
 +
<tr>
 +
<th>SynTFs </th>
 +
<th>SynPros</th>
 +
</tr>
 +
<tr>
 +
<td>Gal4-KRAB(TF-KRAB-1)  <partinfo>BBa_K2446037</partinfo></td>
 +
<td>Sv40-UAS(Sv40-UAS) <partinfo>BBa_K2446036</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_PIP_KRAB(TF-KRAB-2) <partinfo>BBa_K2446045</partinfo></td>
 +
<td>SV40_2/4/8_PIP <partinfo>BBa_K2446033</partinfo>/<partinfo>BBa_K2446034</partinfo>/<partinfo>BBa_K2446035</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_21-16KRAB(TF-KRAB-3) <pratinfo>BBa_K2446039 </pratinfo></td>
 +
<td>SV40_8_ZF_21-16 <pratinfo>BBa_K2446030</pratinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_42-10_KRAB(TF-KRAB-4) <pratinfo>BBa_K2446040</pratinfo></td>
 +
<td>SV40_8_ZF_42-10 <pratinfo>BBa_K2446025</pratinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_43-8_KRAB(TF-KRAB-5) <pratinfo>BBa_K2446041</pratinfo></td>
 +
<td>SV40_2/4/8_ZF_43-8 <pratinfo>BBa_K2446026</pratinfo>/<partinfo>BBa_K2446027</partinfo>/<partinfo>BBa_K2446028</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_54-8_KRAB(TF-KRAB-6) <pratinfo>BBa_K2446042</pratinfo></td>
 +
<td>SV40_8_ZF_54-8 <pratinfo>BBa_K2446029</pratinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZFHD1_KRAB(TF-KRAB-7) <pratinfo>BBa_K2446043</pratinfo></td>
 +
<td>SV40_4_ZFHD1 <pratinfo>BBa_K2446032</pratinfo></td>
 +
</tr>
 +
</table>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:38, 29 October 2017


ZF_GAl4_KRAB

This part is one of our mammalian synthetic transcription factors (SynTFs) based on Gal4 DNA binding domain [1]. Gal4-KRAB (or TF-KRAB-1 in our project) containing three core domains from N-terminal to C-terminal: GAL4 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [2].And a (G4S) linker was added between DBD and NLS for providing region flexibility [3]. GAL4DBD enable binding to specific DNA sequences, so that we can use Gal4-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter Sv40-UAS (BBa_K2446036).

The information of other SynTF-SynPro is showed in the table below.

SynTFs SynPros
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 Sv40-UAS(Sv40-UAS) BBa_K2446036
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035
ZF_21-16KRAB(TF-KRAB-3) <pratinfo>BBa_K2446039 </pratinfo> SV40_8_ZF_21-16 <pratinfo>BBa_K2446030</pratinfo>
ZF_42-10_KRAB(TF-KRAB-4) <pratinfo>BBa_K2446040</pratinfo> SV40_8_ZF_42-10 <pratinfo>BBa_K2446025</pratinfo>
ZF_43-8_KRAB(TF-KRAB-5) <pratinfo>BBa_K2446041</pratinfo> SV40_2/4/8_ZF_43-8 <pratinfo>BBa_K2446026</pratinfo>/BBa_K2446027/BBa_K2446028
ZF_54-8_KRAB(TF-KRAB-6) <pratinfo>BBa_K2446042</pratinfo> SV40_8_ZF_54-8 <pratinfo>BBa_K2446029</pratinfo>
ZFHD1_KRAB(TF-KRAB-7) <pratinfo>BBa_K2446043</pratinfo> SV40_4_ZFHD1 <pratinfo>BBa_K2446032</pratinfo>

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 256
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 256
    Illegal NgoMIV site found at 41
    Illegal NgoMIV site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Morsut, L. et al. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).

[2] Witzgall, R., O'Leary, E., Leaf, A., Onaldi, D. & Bonventre, J. V. The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).

[3] Chen, X., Zaro, J. L. & Shen, W.-C. Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews 65, 1357-1369 (2013).