Difference between revisions of "Part:BBa K2325000"
 
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The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.  
 
The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.  
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<br />
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2325000 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
 
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
 
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
  
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<b>formamidase gene</b>
 
 
<center><h4>HCO - <font color="red">NH<sub>2</sub></font> + H<sub>2</sub>O &rArr; HCOOH + <font color="red">NH<sub>3</sub></font></h4></center>
 
<center><h4>HCO - <font color="red">NH<sub>2</sub></font> + H<sub>2</sub>O &rArr; HCOOH + <font color="red">NH<sub>3</sub></font></h4></center>
  
 
===Gel analysis===
 
===Gel analysis===
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[[File:BBa_K2325000--1.png]]
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[[File:BBa_K2325000--1.png|300px]]
  
 
===Enzymatic properties===
 
===Enzymatic properties===
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<center>
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[[File:BBa_K2325000--2.png|850px]]<br />
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[[File:BBa_K2325000--2.png|thumb|left|600px|'''Figure 1''': Enzymatic activity of formamidase under different temperatures and pH]]<br />
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<b>Figure 1.</b>Enzymatic activity of formamidase under different temperatures and pH
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</center>
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<br><table>
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<tr><th>
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<center>
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[[File:BBa_K2325000--3.png|thumb|left|550px|'''Figure 2''': The Michaelis constant of formamidase]]</th><th>
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[[File:BBa_K2325000--3.png|750px]]<br />
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[[File:BBa K2325000--5.png|thumb|right|330px|'''Figure 3''': Influence of metal ion on the enzymatic activity of formamidase]]<br /></th></tr></table>
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<b>Figure 2.</b>The Michaelis constant of formamidase
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[[File:BBa_K2325000--4.png|thumb|left|350px|]]<br />
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</center>
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<br />
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<center>
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[[File:BBa_K2325000--4.png|500px]]<br />
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[[File:BBa K2325000--5.png]]<br />
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<b>Figure 3.</b>Influence of metal ion on the enzymatic activity of formamidase
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</center>
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<br />
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2325000 SequenceAndFeatures</partinfo>
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Latest revision as of 15:07, 29 October 2017


Formamidase (FOR)

The BBa_K2325000 contains a coding sequence of formamidase which catalyzes the conversion of formamide to ammonia.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.

HCO - NH2 + H2O ⇒ HCOOH + NH3

Gel analysis

BBa K2325000--1.png

Enzymatic properties

Figure 1: Enzymatic activity of formamidase under different temperatures and pH


Figure 2: The Michaelis constant of formamidase
Figure 3: Influence of metal ion on the enzymatic activity of formamidase

BBa K2325000--4.png