Difference between revisions of "Part:BBa K2446037"

 
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This part is one of our mammalian synthetic transcription factors (SynTFs) based on Gal4 DNA binding domain [[#References|[1]]]. Gal4-KRAB (or TF-KRAB-1 in our project) containing three core domains from N-terminal to C-terminal: GAL4 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [[#References|[2]]].And a (G4S) linker was added between DBD and NLS for providing region flexibility [[#References|[3]]]. GAL4DBD enable binding to specific DNA sequences, so that we can use Gal4-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter Sv40-UAS <partinfo>( BBa_K2446036)</partinfo>.
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The information of other SynTF-SynPro is showed in the table below.
  
 
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<partinfo>BBa_K2446037 parameters</partinfo>
 
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===References===
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[1] Morsut, L. et al. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).
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[2] Witzgall, R., O'Leary, E., Leaf, A., Onaldi, D. & Bonventre, J. V. The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).
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[3] Chen, X., Zaro, J. L. & Shen, W.-C. Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews 65, 1357-1369 (2013).

Revision as of 15:04, 29 October 2017


ZF_GAl4_KRAB

This part is one of our mammalian synthetic transcription factors (SynTFs) based on Gal4 DNA binding domain [1]. Gal4-KRAB (or TF-KRAB-1 in our project) containing three core domains from N-terminal to C-terminal: GAL4 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [2].And a (G4S) linker was added between DBD and NLS for providing region flexibility [3]. GAL4DBD enable binding to specific DNA sequences, so that we can use Gal4-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter Sv40-UAS No part name specified with partinfo tag.. The information of other SynTF-SynPro is showed in the table below.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 256
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 256
    Illegal NgoMIV site found at 41
    Illegal NgoMIV site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Morsut, L. et al. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016). [2] Witzgall, R., O'Leary, E., Leaf, A., Onaldi, D. & Bonventre, J. V. The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994). [3] Chen, X., Zaro, J. L. & Shen, W.-C. Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews 65, 1357-1369 (2013).