Difference between revisions of "Part:BBa K2448022"

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D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens str. C58 (UniProt A9CH28)
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This is the original sequence of the D-Psicose 3-epimerase (Dpe) from ''Agrobacterium tumefaciens'' str. C58 (Atu4750, UniProt A9CH28).
  
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===Usage and Biology===
 
===Usage and Biology===
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D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-Psicose being the carbon-3 (C3) epimer of D-fructose [1].
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[File: T--Evry_Paris-Saclay--EC 5.1.3.30.png]
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This enzyme is highly specific for D-Psicose, but is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose, and D-sorbose [1].
  
 
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Revision as of 14:40, 29 October 2017


D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens

This is the original sequence of the D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens str. C58 (Atu4750, UniProt A9CH28).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-Psicose being the carbon-3 (C3) epimer of D-fructose [1].

[File: T--Evry_Paris-Saclay--EC 5.1.3.30.png]

This enzyme is highly specific for D-Psicose, but is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose, and D-sorbose [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 601
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 156