Difference between revisions of "Part:BBa K2448008"
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<partinfo>BBa_K2448008 short</partinfo> | <partinfo>BBa_K2448008 short</partinfo> | ||
| − | This part is an E. coli codon optimized version of the PsiR found in ''Sinorhizobium meliloti'' (SMb20483). | + | This part is an ''E. coli'' codon optimized version of the PsiR found in ''Sinorhizobium meliloti'' (SMb20483). |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:05, 29 October 2017
PsiR from Sinorhizobium meliloti
This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium meliloti (SMb20483).
Usage and Biology
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
Our team used this transcription factor together with the Sinorhizobium meliloti pPsiA or pPsiR promoters (BBa_K2448014 and BBa_K2448015) to build two Psicose biosensors (BBa_K2448030 and BBa_K2448031). The results show that PsiR behaved as predicted under and without induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 691
Illegal AgeI site found at 1024 - 1000COMPATIBLE WITH RFC[1000]