Difference between revisions of "Part:BBa K2448006"
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<partinfo>BBa_K2448006 short</partinfo> | <partinfo>BBa_K2448006 short</partinfo> | ||
| − | This part is an E. coli codon optimized version of the PsiR found in ''Agrobacterium tumefaciens'' str. C58 (Atu4743). | + | This part is an ''E. coli'' codon optimized version of the PsiR found in ''Agrobacterium tumefaciens'' str. C58 (Atu4743). |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:04, 29 October 2017
PsiR from Agrobacterium tumefaciens
This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).
Usage and Biology
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
Our team used this transcription factor together with the Agrobacterium tumefaciens pPsiA or pPsiR promoters (BBa_K2448010 and BBa_K2448011) as well as with the pPsiTac1 synthetic promoter (BBa_K2448016) to build three Psicose biosensors (BBa_K2448025, BBa_K2448026 and BBa_K2448027). The results show that PsiR behaved as predicted under and without induction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4
Illegal AgeI site found at 691 - 1000COMPATIBLE WITH RFC[1000]