Difference between revisions of "Part:BBa K2305010"

Latest revision as of 11:47, 29 October 2017

GFP/RFP dual fluorescent system with LacI and plac

It's a GFP/RFP dual fluorescent system

Agarose gel electrophoresis

We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

DNA sequencing[edit] The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

Characterization map

Fig.The Growth curve of E.coil.

Fig.The GFP curve of E.coil.

Fig.The RFP curve of E.coil.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1165
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2846
Illegal AgeI site found at 2958
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1844

@@ Line 5: / Line 5: @@
 It's a GFP/RFP dual fluorescent system
 ==Agarose gel electrophoresis==
-We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
+We calculated the new part’s base number to be 3134bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 [[Image:BIT Figure DNA sequencing K2305010.png|200px|left|K230500]]<br clear="all">
@@ Line 13: / Line 13: @@
 [[Image:BIT Figure DNA sequencing K2305010b.png|200px|left|K230500]]
 <br clear="all">
+==Characterization map==
+[[Image:BIT Figure map K2305010.png|400px|left|K230500]]<br clear="all">
+Fig.The Growth curve of E.coil.
+[[Image:BIT Figure map K2305010a.png|400px|left|K230500]]<br clear="all">
+Fig.The GFP curve of E.coil.
+[[Image:BIT Figure map K2305010b.png|400px|left|K230500]]<br clear="all">
+Fig.The RFP curve of E.coil.