Difference between revisions of "Part:BBa K2305002"
 
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promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
 
promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
  
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===DNA sequencing===
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===Agarose gel electrophoresis===
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.
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The figure is a comparison with the results of the Agarose gel electrophoresis. It verified the accuracy of the sequence further.
  
 
[[Image:BIT Figure DNA sequencing K2305002.PNG|200px|left|K2305002]]<br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305002.PNG|200px|left|K2305002]]<br clear="all">

Latest revision as of 11:45, 29 October 2017


pbad promter with GFP/RFP dual fluorescent system

promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.

Agarose gel electrophoresis

The figure is a comparison with the results of the Agarose gel electrophoresis. It verified the accuracy of the sequence further.

K2305002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1303
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2984
    Illegal AgeI site found at 3096
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1982