Difference between revisions of "Part:BBa K2305002"
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promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally. | promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally. | ||
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| + | ===Agarose gel electrophoresis=== | ||
| + | The figure is a comparison with the results of the Agarose gel electrophoresis. It verified the accuracy of the sequence further. | ||
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| + | [[Image:BIT Figure DNA sequencing K2305002.PNG|200px|left|K2305002]]<br clear="all"> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 11:45, 29 October 2017
pbad promter with GFP/RFP dual fluorescent system
promoter pbad start the line, the lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
Agarose gel electrophoresis
The figure is a comparison with the results of the Agarose gel electrophoresis. It verified the accuracy of the sequence further.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1303
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2984
Illegal AgeI site found at 3096 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1982