Difference between revisions of "Part:BBa K2406051:Experience"
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| − | + | In our experience, this part worked as expected. Check main page for more information. | |
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===Applications of BBa_K2406051=== | ===Applications of BBa_K2406051=== | ||
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Our Experience shows that that this part works. When transformed into a cell that also had BBa_K2406030 (part that would allow for Dre recombinase expression), we could assess function of Dre recombinase using this part. We measured RFP fluorescence over the course of a several hours. One control was not induced with IPTG, meaning we expected there to be no Dre recombinase produced. This was compared to a culture that was induced with IPTG. Based on our assay, there is a clear difference between fluorescent output in induced and un-induced cultures (averaged over 8 replicates) confirming that both this part and BBa_K2406030 work as expected. | Our Experience shows that that this part works. When transformed into a cell that also had BBa_K2406030 (part that would allow for Dre recombinase expression), we could assess function of Dre recombinase using this part. We measured RFP fluorescence over the course of a several hours. One control was not induced with IPTG, meaning we expected there to be no Dre recombinase produced. This was compared to a culture that was induced with IPTG. Based on our assay, there is a clear difference between fluorescent output in induced and un-induced cultures (averaged over 8 replicates) confirming that both this part and BBa_K2406030 work as expected. | ||
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| + | [[Image:BBa_K2406051-graph_1.png|200px|thumb|left|Graph showing RFP expression (measured by fluorescence) as a result of Dre expression compared to uninduced control]] | ||
Latest revision as of 10:39, 29 October 2017
In our experience, this part worked as expected. Check main page for more information.
Applications of BBa_K2406051
Our team used this part to measure the activity of Dre recombinase. This is because if Dre recombinase was in the cell, it would be cause recombination between the two Rox sites, leading to excision of the terminator, thus causing RFP to be expressed. Therefore, this construct allowed for an assay of Dre recombinase activity.
User Reviews
UNIQ7f53c3519e87fd05-partinfo-00000000-QINU UNIQ7f53c3519e87fd05-partinfo-00000001-QINU
Our Experience shows that that this part works. When transformed into a cell that also had BBa_K2406030 (part that would allow for Dre recombinase expression), we could assess function of Dre recombinase using this part. We measured RFP fluorescence over the course of a several hours. One control was not induced with IPTG, meaning we expected there to be no Dre recombinase produced. This was compared to a culture that was induced with IPTG. Based on our assay, there is a clear difference between fluorescent output in induced and un-induced cultures (averaged over 8 replicates) confirming that both this part and BBa_K2406030 work as expected.
