Difference between revisions of "Part:BBa K2271105:Experience"
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===Applications of BBa_K2271105=== | ===Applications of BBa_K2271105=== | ||
| − | ===Import of fluorescent proteins into the peroxisomal matrix=== | + | ====Import of fluorescent proteins into the peroxisomal matrix==== |
| − | [[Image:Artico_15wtp13.png|frame|'''Figure 1:'''PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1 | + | [[Image:Artico_15wtp13.png|frame|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]] |
| − | + | ||
| − | + | ||
| + | [[Image:Artico_15p3p13.png|frame|center|'''Figure 2:'''PEX5 knock out strain, transformed with the R15 variant, mTurqouise tagged with the PTS1* P3 and the PEX13 membrane anchor fused to mRuby.]] | ||
| + | <p align="justify"> | ||
| + | The figures 1 and 2 show two different experiments. The first in figure 1 is the control if the PEX5 variant R15 imports proteins tagged with the natural PTS1 and the second in figure 2 is an actual experiment if the variant imports proteins tagged with one of our new PTS1* variants − in this case it was P3 (-YTNQE). One can see that there is no import in both cases. While PEX13-mRuby marks the peroxisomal membrane, mTurqouise is located in the cytosol − this indicates that there is no import. | ||
| + | <br> | ||
| + | We wanted to make a screening for functional PTS1* variants but unfortuneately the Violacein assay planned for that (see our [http://2017.igem.org/Team:Cologne-Duesseldorf/Design#h3-3 wiki article] for more information) did not work out. As outlook we propose to find a fitting PTS1* hence the molecular dynamics simulations showed promising results. | ||
| + | |||
| + | </p> | ||
<br> | <br> | ||
Latest revision as of 10:17, 29 October 2017
Applications of BBa_K2271105
Import of fluorescent proteins into the peroxisomal matrix
The figures 1 and 2 show two different experiments. The first in figure 1 is the control if the PEX5 variant R15 imports proteins tagged with the natural PTS1 and the second in figure 2 is an actual experiment if the variant imports proteins tagged with one of our new PTS1* variants − in this case it was P3 (-YTNQE). One can see that there is no import in both cases. While PEX13-mRuby marks the peroxisomal membrane, mTurqouise is located in the cytosol − this indicates that there is no import.
We wanted to make a screening for functional PTS1* variants but unfortuneately the Violacein assay planned for that (see our [http://2017.igem.org/Team:Cologne-Duesseldorf/Design#h3-3 wiki article] for more information) did not work out. As outlook we propose to find a fitting PTS1* hence the molecular dynamics simulations showed promising results.
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